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KMID : 1059519920360060849
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Journal of the Korean Chemical Society
1992 Volume.36 No. 6 p.849 ~ p.856
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Centrifugal Liquid Chromatography with Application of the N-hexadecyl-L-proline Coated Reversed Phase for Separation of amino Acid Enantiomers
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Lee Sun-Haing
Oh Tae-Sub
Lee Hae-Woon
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Abstract
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We prepared a simple, economic and versatile preparative system for an enantiomeric separation. Hydrophobic amino acid enantiomers were resolved in the preparative scale by a centrifugal liquid chromatography on the N-hexadecyl-L-proline coated reversed phase. The factors controlling the retention and resolution of racemic amino acids such as the concentration of Cu(Ⅱ), pH of the eluent, the type and content of organic modifier, and rpm of CLC were examined. Several mg of hydrophobic amino acid enantiomers were separated preparatively. To separate all of different amino acid enantiomers, various coating material will be investigated.
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KEYWORD
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